ABSTRACT
A detecçäo de Toxoplasma gondii no sangue venoso e na placenta de gestantes pela reaçäo de polimerase em cadeia pode facilitar o diagnóstico préðnatal do toxoplasmose congênita. Foram avaliadas gestantes IgMðreagentes e os seus filhos. Além das dosagens de IgG, IgM, IgA e reaçäo de avidez de IgG (MEIA), foram realizadas a técnica de imunoperoxidase e a inoculaçäo em camundongos. De cada amostra foi efetuada amplificaçäo gênica com primers do gene B1 e novos primers do gene TGR (chamados ABGTg7 C1 e N1). É preciso observar que o tratamento poderia ser responsável por uma diminuiçäo da infecçäo. Desta forma, o diagnóstico negativo confirmaria a eficiência do tratamento preventivo na replicaçäo parasitária no útero. A reaçäo de polimerase em cadeia mostrouðse sensível e específica; evidenciou a presença de um a dez taquizoítas; pode ser utilizada com segurança e confiabilidade, além de tornar rápido o diagnóstico da toxoplasmose congênita, sendo, assim, ferramenta importante na avaliaçäo préðnatal
Subject(s)
Humans , Female , Pregnancy , Fetal Diseases/diagnosis , Fetal Diseases/parasitology , Fetal Blood , Immunoenzyme Techniques , Placenta , Polymerase Chain Reaction , Pregnancy , Pregnancy Complications, Parasitic , Prenatal Diagnosis , Sensitivity and Specificity , Toxoplasma , Toxoplasmosis, Congenital/diagnosis , Toxoplasmosis, Congenital/bloodABSTRACT
The aim of this case series was to describe the clinical, laboratory and epidemiological characteristics and the presentation of bacillary angiomatosis cases (and/or parenchymal bacillary peliosis) that were identified in five public hospitals of Rio de Janeiro state between 1990 and 1997; these cases were compared with those previously described in the medical literature. Thirteen case-patients were enrolled in the study; the median age was 39 years and all patients were male. All patients were human immunodeficiency virus type 1 (HIV-1) infected and they had previous or concomitant HIV-associated opportunistic infections or malignancies diagnosed at the time bacillary angiomatosis was diagnosed. Median T4 helper lymphocyte counts of patients was 96 cells per mmÝ. Cutaneous involvement was the most common clinical manifestation of bacillary angiomatosis in this study. Clinical remission following appropriate treatment was more common in our case series than that reported in the medical literature, while the incidence of relapse was similar. The frequency of bacillary angiomatosis in HIV patients calculated from two of the hospitals included in our study was 1.42 cases per 1000 patients, similar to the frequencies reported in the medical literature. Bacillary angiomatosis is an unusual opportunistic pathogen in our setting